Compared to any other source, the vast amounts of adipose tissue (depots of fat for storing energy) especially in the abdominal region, by sheer volume of availability, ensure an abundance in numbers of regenerative cells ranging in the millions per unit volume. The sheer numbers available also has the added advantage of not needing to be cultured in a laboratory over days in order to get the desired number of regenerative cells to achieve what is called “therapeutic threshold” i.e. therapeutic benefit. In addition, harvesting regenerative cells from adipose tissue through simple, minimally invasive liposuction under local anesthesia is relatively easier, painless and poses minimal risk to the patient compared to all other possible methods.
Our process yields millions upon millions of the Vascular Fraction Cells. VFC cells include autologous pluripotent adult stem cells, endothelial cells, progenitor cells, fibroblast cells, red blood cells, white blood cells, and many growth factors found in the vascular tissue that is the basis for the Vascular Fraction Cells.
VFC cellular composition: Adult autologous stem cells (Mesenchymal) Endothelial Cells Fibroblasts Growth Factors Pericytes Pre-adipocytes cells Smooth muscle cells
Blood Cells from the capillaries supply including: B&T cells Erythrocytes Hematopoietic stem cells Endothelial progenitor cells Macrophages Mast Cells Monocytes Natural killer (NK) cells
The patient visiting the clinic will receive a mini-liposuction procedure, under local anesthetic, and the physician will remove approximately 60 cc's of adipose (fat) tissue from the abdomen. Adipose tissue is primarily composed of the adipocyte tissue (80%) and a network of mostly capillaries that surround the adipocytes.
The IntelliCell™ process uses ultrasound to separate the network of capillaries from the adipocytes. In a closed sterile process that is very similar to obtaining cells from bone marrow, the vascular tissue after it has been separated from the adipocytes, is washed in a sterile area and placed in a centrifuge and spun at low levels for several minutes. The actual fat tissue that was obtained via the liposuction procedure is discarded. The autologous vascular cells drop to the bottom of the collection container and are prepared for quality testing.
Unlike most companies that use other types of equipment, We / IntelliCell™ uses a flow cytometer to check each sample for cell viability and the cell count for each patient. This is an important quality step that permits us to generate the safety data that is required to adhere to good laboratory practices.
The entire process takes about 1 hour to complete. Some of the cells are placed into an IV drip bag for administration. The IV treatment takes about 20 minutes. Depending on the areas being treated and the type of injury, cells can be also be placed locally. For example, a case of Osteoarthritis will have an injection of the cells directly into the joint.
Our physicians will administer the stem cells via a combination of routes: